Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts
نویسندگان
چکیده
In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts used to assess bioactivities plant. The antimicrobial activity was tested against a panel Gram-positive -negative pathogenic bacterial fungal strains using microdilution method. cytotoxicity K. investigated human-derived macrophage THP-1 cells through MTT assay. Finally, anti-inflammatory studied same cell line by measuring secretion IL-10 IL-6. phytoconstituents hexane chloroform evaluated gas chromatography–mass spectrometry (GC/MS). addition, high-performance liquid chromatography (HPLC) study phytochemical content methanol extract. total flavonoid (TFC) extract is also reported. chemical composition Fourier-transform infrared spectroscopy (FTIR). Results revealed that inhibited growth Pseudomonas aeruginosa at 150 μg/mL. At concentration, methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, three highly cytotoxic IC50 < 3 significantly stimulated 50 μg/mL (p 0.01). GC/MS analyses contain fatty acids, sterols, vitamin E, triterpenes. HPLC analysis demonstrated constituted quercetin kaempferol derivatives. This first report in which profiles are assessed for non-polar polar extracts.
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ژورنال
عنوان ژورنال: Plants
سال: 2023
ISSN: ['2223-7747']
DOI: https://doi.org/10.3390/plants12101943